Facilities
The Computer-Aided Molecular Design laboratory of Yuan-Ping Pang, Ph.D., is located on the 12th floor of the Stabile Building on the Mayo Clinic campus in Rochester, Minn. Laboratory areas include:
- A computational chemistry unit (1,342 square feet)
- A synthetic chemistry unit with 14 6-foot fume hoods (3,002 square feet)
- A bioassay lab (88 square feet)
Equipment
Equipment for synthesis and characterization of compounds includes:
- Varian 400 MHz nuclear magnetic resonance (NMR) system dedicated to Dr. Pang's lab, featuring a 1H/19F/X 5 mm autotune dual broadband probe, 1H-19F[15N-31P] 3 mm indirect broadband probe, 23 channel shims and shim supply, variable temperature module, and 100-sample autosampler
- Thermo Nicolet Avatar 370 Fourier transform infrared spectrometer (FT-IR)
- Jasco DIP-370 digital polarimeter
- Molecular Devices SpectraMax Plus384 microplate reader
- Three high-performance liquid chromatography (HPLC) systems (Beckman System Gold 125P solvent module and 166P UV detector)
- Agilent HP 6890 gas chromatograph and Agilent HP5973 mass spectrometer
- Liquid chromatography-mass spectrometry system (Waters 1525 binary HPLC pump, Waters 2487 dual lambda absorbance detector and Waters Micromass ZQ 2000)
- Four Biotage SP1 flash purification systems
- Isco CombiFlash Sg 100 gradient chromatography system
- Isotemp cooling circulator (minus 20 degrees Celsius to 20 degrees Celsius)
- Microcentrifuge, ovens, Kugelrohr distillation apparatus, melting point apparatus, hydrogenator, high-pressure reaction vessels, rotary evaporators, analytical balances, semi-micro balance, ozone generators, temperature controllers and freeze dryer
Equipment for solid-phase combinatorial chemistry using the radiofrequency-encoded split-and-pool technique includes:
- AccuTag-100 system (AccuTag scanning station, Synthesis Manager software and MicroKan decapping tool)
- Forty-eight-position optical sorting indicators
- MultiTag-96 station drops radiofrequency tags into 8x12 arrays of MicroKans or MiniKans
- MultiCap-96 station
- Ninety-six-position dry resin filling station for MicroKans and MiniKans
- Reuseable glass-coated radio frequency tags
- MicroKans, MiniKans and MacroKans reactors
- Genevac DD4 evaporation system
Equipment for parallel synthesis includes:
- Argonaut Quest 210 ASW (equipped for solution- and solid-phase parallel synthesis) with automated solvent wash module and chiller interface kit
- Five mL and 10 mL Teflon reaction vessels with 7 mm and 30 mm frits
- JULABO FP88-MW compact ultra-low refrigerated circulator
Equipment for zebrafish experiments includes:
- Leica M165 FC fluorescent stereo microscope with Leica IC80 HD camera, KL 1500 LCD and ErgoTube 10-50 binocular
- Narishige three-axis course manipulator
- Eppendorf Femtojet microinjector
- Tecniplast ZEBTEC zebrafish housing system with 50 3.5-liter tanks, 15 1.1-liter tanks, a dedicated water treatment unit with automatic pH and conductivity dosing
- Two 100-liter fish tanks (each equipped with one Eheim Classic 2217 external canister filter)
- Noldus EthoVision XT video tracking system for tracking and analyzing the behavior, movement and activity of animals
Equipment for biochemistry and molecular biology includes:
- Benchmark Scientific MyFuge 12 Mini Centrifuge
- Labnet Prism R Refrigerated Microcentrifuge
- Fisher Scientific Marathon Micro A Centrifuge
- Fisher Scientific Centrific Model 228 Benchtop Centrifuge
- Genevac DD-4 centrifugal evaporation system
- Thermo Scientific Owl EasyCast B1 Mini Gel Electrophoresis System combs
- Bio-Rad Mini-Protean Tetra Electrophoresis System
- Bio-Rad MicroPulser electroporator
- Fisher Scientific FB300 power supply
- Bio-Rad PowerPac Basic power supply
- Beckman Coulter ProteomeLab FC/1 module fraction collector
- Fisher Scientific Isotemp Model 202S digital control water bath
- DPC MicroMix Model 5 microplate and tube shaker
- Invitrogen Sample Mixer
- Bioer GenePro thermal cycler
- Eppendorf Research pipettes (sets of 10, 20, 50, 100, 200 and 1000 microliters) and Biohit electronic eLine pipettes
- Summit Commercial Products SCR600L Plus medical refrigerator with glass door
- Molecular Devices SpectraMax Gemini XS microplate spectrofluorometer
- Molecular Devices SpectraMax Plus384 microplate reader
- Biacore 3000 Surface plasmon resonance biomolecular binding system
Dedicated computer hardware includes:
- Kibbutz100 (1.1 teraflops, 470 Xeon processors, 2.2/2.4 GHz, 512 MB memory/node, 8.4 TB disk)
- SilverGene (2.7 teraflops, 590 G5 processors, 2.0/2.3 GHz, 512 MB memory/node, 4 TB Xserve RAID)
- PowerGene (IBM SMP workstation, four POWER5 processors, 1.95 GHz, 36 MB cache, 8 GB DDR2 memory)
- LeopardGene (eight eight-core Mac Pros with Intel Woodcrest 3.0 GHz)
- LionGene (30.0 teraflops, 100 12-core Mac Pros with Intel Westmere 2.40/2.93 GHz)
